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TRPM7 Antibody (aa1817-1863, clone S74-25)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC, IP |
|---|---|
| Primary Accession | Q96QT4 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | S74-25 |
| Calculated MW | 213kDa |
| Dilution | ICC (0.1-1 µg/ml), IHC-P (5 µg/ml), IP (1-10 µg/ml), WB (1-10 µg/ml) , |
| Gene ID | 54822 |
|---|---|
| Other Names | Transient receptor potential cation channel subfamily M member 7, 2.7.11.1, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7, TRPM7, CHAK1, LTRPC7 |
| Target/Specificity | Detects ~220 kD protein. No cross reactivity with TrpM6. |
| Reconstitution & Storage | Store at -20°C. |
| Precautions | TRPM7 Antibody (aa1817-1863, clone S74-25) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRPM7 |
|---|---|
| Synonyms | CHAK1, LTRPC7 {ECO:0000303|PubMed:113855 |
| Function | Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. The channel is highly permeable to divalent cations, specifically calcium (Ca2+), magnesium (Mg2+) and zinc (Zn2+) and mediates their influx (PubMed:11385574, PubMed:12887921, PubMed:15485879, PubMed:24316671, PubMed:35561741, PubMed:36027648). Controls a wide range of biological processes such as Ca2(+), Mg(2+) and Zn(2+) homeostasis, vesicular Zn(2+) release channel and intracellular Ca(2+) signaling, embryonic development, immune responses, cell motility, proliferation and differentiation (By similarity). The C-terminal alpha-kinase domain autophosphorylates cytoplasmic residues of TRPM7 (PubMed:18365021). In vivo, TRPM7 phosphorylates SMAD2, suggesting that TRPM7 kinase may play a role in activating SMAD signaling pathways. In vitro, TRPM7 kinase phosphorylates ANXA1 (annexin A1), myosin II isoforms and a variety of proteins with diverse cellular functions (PubMed:15485879, PubMed:18394644). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q923J1}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q923J1}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q923J1}. Note=Localized largely in intracellular Zn(2+)-storage vesicles. {ECO:0000250|UniProtKB:Q923J1} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
References
Nadler M.J.S.,et al.Nature 411:590-595(2001).
Nadler M.J.S.,et al.Nature 412:660-660(2001).
Ryazanova L.V.,et al.J. Biol. Chem. 279:3708-3716(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schmitz C.,et al.Cell 114:191-200(2003).
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