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SMO / Smoothened Antibody (aa68-117)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q99835 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 86kDa |
| Dilution | ELISA (1:10000), IHC-P (10 µg/ml), WB (1:500-1:1000) |
| Gene ID | 6608 |
|---|---|
| Other Names | Smoothened homolog, SMO, Protein Gx, SMO, SMOH |
| Target/Specificity | SMO Antibody detects endogenous levels of total SMO protein. |
| Reconstitution & Storage | Store at -20°C for up to one year. |
| Precautions | SMO / Smoothened Antibody (aa68-117) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SMO |
|---|---|
| Synonyms | SMOH |
| Function | G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P56726}; Multi-pass membrane protein. Cell projection, cilium. Note=Cilium localization is promoted by SHH and is required for activity. {ECO:0000250|UniProtKB:P56726} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
References
Stone D.M.,et al.Nature 384:129-134(1996).
Xie J.,et al.Nature 391:90-92(1998).
Jiang N.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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