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SMO / Smoothened Antibody (aa68-117)

Rabbit Polyclonal Antibody

     
  • WB - SMO / Smoothened Antibody (aa68-117) ALS13686
    Western blot of extracts from HUVEC cells, using SMO Antibody.
    detail
  • IHC - SMO / Smoothened Antibody (aa68-117) ALS13686
    Anti-SMO antibody IHC of human lung.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q99835
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 86kDa
Dilution ELISA (1:10000), IHC-P (10 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 6608
Other Names Smoothened homolog, SMO, Protein Gx, SMO, SMOH
Target/Specificity SMO Antibody detects endogenous levels of total SMO protein.
Reconstitution & Storage Store at -20°C for up to one year.
PrecautionsSMO / Smoothened Antibody (aa68-117) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMO
Synonyms SMOH
Function G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P56726}; Multi-pass membrane protein. Cell projection, cilium. Note=Cilium localization is promoted by SHH and is required for activity. {ECO:0000250|UniProtKB:P56726}
Research Areas
Citations (0)
citation

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Background

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.

References

Stone D.M.,et al.Nature 384:129-134(1996).
Xie J.,et al.Nature 391:90-92(1998).
Jiang N.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 467.50
Cat# ALS13686
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