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ENO1 / Alpha Enolase Antibody (clone 8G8)

Mouse Monoclonal Antibody

     
  • WB - ENO1 / Alpha Enolase Antibody (clone 8G8) ALS13306
    Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
    detail
  • WB - ENO1 / Alpha Enolase Antibody (clone 8G8) ALS13306
    ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
    detail
  • IF - ENO1 / Alpha Enolase Antibody (clone 8G8) ALS13306
    Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
    detail
  • IHC - ENO1 / Alpha Enolase Antibody (clone 8G8) ALS13306
    Anti-ENO1 antibody IHC of human breast.
    detail
  • IHC - ENO1 / Alpha Enolase Antibody (clone 8G8) ALS13306
    Anti-ENO1 antibody IHC of human pancreas.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P06733
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8G8
Calculated MW 47kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 2023
Other Names Alpha-enolase, 4.2.1.11, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsENO1 / Alpha Enolase Antibody (clone 8G8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO1
Synonyms ENO1L1, MBPB1, MPB1
Function Glycolytic enzyme the catalyzes the conversion of 2- phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
Cellular Location Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas
Citations (0)
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Background

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

References

Giallongo A.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:6741-6745(1986).
Giallongo A.,et al.Eur. J. Biochem. 190:567-573(1990).
Ray R.,et al.Mol. Cell. Biol. 11:2154-2161(1991).
Walter M.,et al.J. Autoimmun. 8:931-945(1995).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 533.50
Cat# ALS13306
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