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CLIC1 / NCC27 Antibody (clone 2D4)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O00299 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2D4 |
| Calculated MW | 27kDa |
| Dilution | IHC-P (2.5 µg/ml) |
| Gene ID | 1192 |
|---|---|
| Other Names | Chloride intracellular channel protein 1, Chloride channel ABP, Nuclear chloride ion channel 27, NCC27, Regulatory nuclear chloride ion channel protein, hRNCC, CLIC1, G6, NCC27 |
| Reconstitution & Storage | Store at -20°C. Aliquot to avoid freeze/thaw cycles. |
| Precautions | CLIC1 / NCC27 Antibody (clone 2D4) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLIC1 {ECO:0000303|PubMed:16339885, ECO:0000312|HGNC:HGNC:2062} |
|---|---|
| Function | In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response (PubMed:25581026, PubMed:37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox- regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle. |
| Cellular Location | Nucleus. Nucleus membrane; Single-pass membrane protein. Cytoplasm. Cell membrane; Single-pass membrane protein. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q6MG61}. Note=Mostly in the nucleus including in the nuclear membrane (PubMed:12681486, PubMed:9139710). Small amount in the cytoplasm and the plasma membrane (PubMed:9139710). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (PubMed:11551966, PubMed:11940526, PubMed:12681486, PubMed:14613939, PubMed:9139710). Might not be present in the nucleus of cardiac cells (By similarity) {ECO:0000250|UniProtKB:Q6MG61, ECO:0000269|PubMed:11551966, ECO:0000269|PubMed:11940526, ECO:0000269|PubMed:12681486, ECO:0000269|PubMed:14613939, ECO:0000269|PubMed:9139710} |
| Tissue Location | Expression is prominent in heart, placenta, liver, kidney and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
References
Valenzuela S.M.,et al.J. Biol. Chem. 272:12575-12582(1997).
Noh Y.H.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Chuang J.Z.,et al.J. Neurosci. 19:2919-2928(1999).
Ribas G.,et al.J. Immunol. 163:278-287(1999).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
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