DIABLO / SMAC Antibody (clone 79-1-83)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
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Primary Accession | Q9NR28 |
Reactivity | Human, Monkey |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 79-1-83 |
Calculated MW | 27kDa |
Dilution | IHC-P (10 µg/ml) |
Gene ID | 56616 |
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Other Names | Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, DIABLO, SMAC |
Target/Specificity | Human and monkey Smac/DIABLO. |
Reconstitution & Storage | Store at -20°C. |
Precautions | DIABLO / SMAC Antibody (clone 79-1-83) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DIABLO (HGNC:21528) |
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Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. |
Cellular Location | Mitochondrion. Cytoplasm, cytosol Note=Released into the cytosol in a PARL-dependent manner when cells undergo apoptosis. |
Tissue Location | Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed |
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Provided below are standard protocols that you may find useful for product applications.
Background
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
References
Du C.,et al.Cell 102:33-42(2000).
Srinivasula S.M.,et al.J. Biol. Chem. 275:36152-36157(2000).
Fu J.,et al.J. Biol. Chem. 278:52660-52672(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).
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