GABRA4 Antibody (Internal)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | P48169 |
Reactivity | Human |
Host | Goat |
Clonality | Polyclonal |
Calculated MW | 62kDa |
Dilution | ELISA (1:16000), IHC-P (2.5 µg/ml), WB (0.1-0.3 µg/ml) |
Gene ID | 2557 |
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Other Names | Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, GABRA4 |
Target/Specificity | Human GABRA4. |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | GABRA4 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GABRA4 (HGNC:4078) |
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Function | Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:35355020). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed:35355020). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed:35355020). GABAARs containing alpha-4 are predominantly extrasynaptic, contributing to tonic inhibition in dentate granule cells and thalamic relay neurons (By similarity). Extrasynaptic alpha-4-containing GABAARs control levels of excitability and network activity (By similarity). GABAAR containing alpha-4-beta-3- delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (PubMed:35355020). |
Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q9D6F4}; Multi-pass membrane protein {ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN5}. Postsynaptic cell membrane; Multi-pass membrane protein {ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN5} |
Tissue Location | Expressed in the brain. {ECO:0000250|UniProtKB:Q9D6F4} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
References
Yang W.,et al.Eur. J. Pharmacol. 291:319-325(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
McLean P.J.,et al.Genomics 26:580-586(1995).
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