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NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6)

Mouse Monoclonal Antibody

     
  • IHC - NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) ALS12559
    Anti-NT5E / CD73 antibody IHC of human adrenal.
    detail
  • IHC - NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) ALS12559
    Anti-NT5E / CD73 antibody IHC of human pancreas.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P21589
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2B6
Calculated MW 63kDa
Dilution IHC-P (10 µg/ml), WB (1:1000-1:2000),
Additional Information
Gene ID 4907
Other Names 5'-nucleotidase, 5'-NT, 3.1.3.5, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Reconstitution & Storage Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsNT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NT5E
Synonyms NT5, NTE
Function Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor
Research Areas
Citations (0)
citation

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Background

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

References

Misumi Y.,et al.Eur. J. Biochem. 191:563-569(1990).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Mungall A.J.,et al.Nature 425:805-811(2003).
Hansen K.R.,et al.Gene 167:307-312(1995).
Zanoni L.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.

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$ 467.50
Cat# ALS12559
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