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HEC1 / NDC80 Antibody (C-Terminus)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O14777 |
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Calculated MW | 74kDa |
| Dilution | ELISA (1:32000), IHC-P (5 µg/ml), WB (0.5-2 µg/ml) |
| Gene ID | 10403 |
|---|---|
| Other Names | Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2 |
| Target/Specificity | Human NDC80 / HEC1. |
| Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
| Precautions | HEC1 / NDC80 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NDC80 |
|---|---|
| Synonyms | HEC, HEC1, KNTC2 |
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912, PubMed:9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:23891108, PubMed:25743205). |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase (PubMed:14699129) Localizes specifically to the outer plate of the kinetochore (PubMed:14699129). |
| Volume | 250 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
References
Chen Y.,et al.Mol. Cell. Biol. 17:6049-6056(1997).
Chen Y.,et al.J. Biol. Chem. 272:24081-24087(1997).
Zheng L.,et al.Mol. Cell. Biol. 19:5417-5428(1999).
Zheng L.,et al.Mol. Cell. Biol. 20:3529-3537(2000).
Chen Y.,et al.J. Biol. Chem. 277:49408-49416(2002).
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