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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ubiquitin E3 Ligases (RING Domain) Antibodies   >   TRIM25 Antibody (C-Terminus)   

TRIM25 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IF - TRIM25 Antibody (C-Terminus) ALS12401
    Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/ml.
    detail
  • IHC - TRIM25 Antibody (C-Terminus) ALS12401
    Anti-TRIM25 antibody IHC of human brain, cortex.
    detail
  • ICC - TRIM25 Antibody (C-Terminus) ALS12401
    Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/ml.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E
Primary Accession Q14258
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 71kDa
Dilution ICC (20 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml)
Additional Information
Gene ID 7706
Other Names E3 ubiquitin/ISG15 ligase TRIM25, 6.3.2.19, 6.3.2.n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147
Target/Specificity a 19 amino acid peptide from near the carboxy terminus of human TRIM25
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsTRIM25 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM25
Synonyms EFP {ECO:0000303|PubMed:8248217}, RNF147
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599). Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1 (PubMed:17392790, PubMed:29357390, PubMed:30193849, PubMed:31710640, PubMed:33849980, PubMed:36045682). Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712). Mediates 'Lys-63'- linked polyubiquitination of IFIH1 (PubMed:30193849). Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755). Mediates estrogen action in various target organs (PubMed:22452784). Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784). Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity). Plays an essential role in the antiviral activity of ZAP/ZC3HAV1; an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'- linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA (PubMed:28060952, PubMed:28202764). Mediates also the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability (PubMed:36067236).
Cellular Location Cytoplasm. Cytoplasm, Stress granule. Nucleus {ECO:0000250|UniProtKB:Q61510}
Tissue Location Expressed in breast tumors (at protein level). Ubiquitous.
Research Areas
Citations (0)
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Background

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

References

Inoue S.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11117-11121(1993).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Shimada N.,et al.Mol. Cell. Endocrinol. 218:147-153(2004).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).
Nakasato N.,et al.Biochem. Biophys. Res. Commun. 351:540-546(2006).

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$ 467.50
Cat# ALS12401
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