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TLR8 Antibody (aa750-850, clone 44C143)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC |
|---|---|
| Primary Accession | Q9NR97 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 44C143 |
| Calculated MW | 120kDa |
| Dilution | IHC-P (5 µg/ml), WB (1-3 µg/ml), |
| Gene ID | 51311 |
|---|---|
| Other Names | Toll-like receptor 8, CD288, TLR8 |
| Target/Specificity | A KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850. It will cross-react with mouse TLR8. |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
| Precautions | TLR8 Antibody (aa750-850, clone 44C143) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TLR8 (HGNC:15632) |
|---|---|
| Function | Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed:25297876, PubMed:32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed:31778653). Recognizes GU-rich single- stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV- 1 viruses (PubMed:33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed:23520111, PubMed:25599397, PubMed:26929371, PubMed:33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF- kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed:16737960, PubMed:17932028, PubMed:29155428). |
| Cellular Location | Endosome membrane; Single-pass type I membrane protein. Note=Endosomal localization confers distinctive proteolytic processing |
| Tissue Location | Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells. |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
References
Du X.,et al.Eur. Cytokine Netw. 11:362-371(2000).
Chuang T.-H.,et al.Eur. Cytokine Netw. 11:372-378(2000).
Nakajima T.,et al.Immunogenetics 60:727-735(2008).
Georgel P.,et al.PLoS ONE 4:E7803-E7803(2009).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
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