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SPP1 / Osteopontin Antibody (N-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P10451 |
| Reactivity | Human, Mouse, Rat, Pig, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 35kDa |
| Dilution | ELISA (1:20000-1:100000), IHC-P (1:500), WB (1:500-1:2000) |
| Gene ID | 6696 |
|---|---|
| Other Names | Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN |
| Target/Specificity | Synthetic peptide C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Precautions | SPP1 / Osteopontin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPP1 |
|---|---|
| Synonyms | BNSP, OPN |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. |
| Cellular Location | Secreted |
| Tissue Location | Detected in cerebrospinal fluid and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717) Bone. Found in plasma. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
References
Kiefer M.C.,et al.Nucleic Acids Res. 17:3306-3306(1989).
Young M.F.,et al.Genomics 7:491-502(1990).
Shiraga H.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992).
Crosby A.H.,et al.Genomics 27:155-160(1995).
Hijiya N.,et al.Biochem. J. 303:255-262(1994).
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