CD66a / CEACAM1 Antibody (Internal)

Anti-CEACAM1 antibody ALS11148 IHC of human colon.

SPECIFICATION
CITATIONS
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	IHC-P
Primary Accession	P13688
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	58kDa
Dilution	IHC-P (5 µg/ml)

Additional Information
Gene ID	634
Other Names	Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, CD66a, CEACAM1, BGP, BGP1
Target/Specificity	Human CEACAM1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Reconstitution & Storage	Long term: -70°C; Short term: +4°C
Precautions	CD66a / CEACAM1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CEACAM1 (HGNC:1814)
Function	[Isoform 1]: Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis (PubMed:18424730, PubMed:23696226, PubMed:25363763). Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils (PubMed:18424730, PubMed:23696226). Upon TCR/CD3 complex stimulation, inhibits TCR- mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70 (PubMed:18424730). Also inhibits T cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T cell through its interaction with HAVCR2 (PubMed:25363763). Upon natural killer (NK) cells activation, inhibit KLRK1-mediated cytolysis of CEACAM1-bearing tumor cells by trans-homophilic interactions with CEACAM1 on the target cell and lead to cis-interaction between CEACAM1 and KLRK1, allowing PTPN6 recruitment and then VAV1 dephosphorylation (PubMed:23696226). Upon neutrophils activation negatively regulates IL1B production by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, that dephosphorylates SYK, reducing the production of reactive oxygen species (ROS) and lysosome disruption, which in turn, reduces the activity of the inflammasome. Down-regulates neutrophil production by acting as a coinhibitory receptor for CSF3R by down-regulating the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R (By similarity). Also regulates insulin action by promoting INS clearance and regulating lipogenesis in liver through regulating insulin signaling (By similarity). Upon INS stimulation, undergoes phosphorylation by INSR leading to INS clearance by increasing receptor-mediated insulin endocytosis. This inernalization promotes interaction with FASN leading to receptor-mediated insulin degradation and to reduction of FASN activity leading to negative regulation of fatty acid synthesis. INSR-mediated phosphorylation also provokes a down-regulation of cell proliferation through SHC1 interaction resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways (By similarity). Functions as activator in angiogenesis by promoting blood vessel remodeling through endothelial cell differentiation and migration and in arteriogenesis by increasing the number of collateral arteries and collateral vessel calibers after ischemia. Also regulates vascular permeability through the VEGFR2 signaling pathway resulting in control of nitric oxide production (By similarity). Down-regulates cell growth in response to EGF through its interaction with SHC1 that mediates interaction with EGFR resulting in decrease coupling of SHC1 to the MAPK3/ERK1- MAPK1/ERK2 pathway (By similarity). Negatively regulates platelet aggregation by decreasing platelet adhesion on type I collagen through the GPVI-FcRgamma complex (By similarity). Inhibits cell migration and cell scattering through interaction with FLNA; interferes with the interaction of FLNA with RALA (PubMed:16291724). Mediates bile acid transport activity in a phosphorylation dependent manner (By similarity). Negatively regulates osteoclastogenesis (By similarity).
Cellular Location	[Isoform 1]: Cell membrane {ECO:0000250\|UniProtKB:P16573}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P16573}. Lateral cell membrane {ECO:0000250\|UniProtKB:P16573}. Apical cell membrane {ECO:0000250\|UniProtKB:P16573}. Basal cell membrane {ECO:0000250\|UniProtKB:P16573}. Cell junction. Cell junction, adherens junction {ECO:0000250\|UniProtKB:P16573}. Note=Canalicular domain of hepatocyte plasma membranes. Found as a mixture of monomer, dimer and oligomer in the plasma membrane. Occurs predominantly as cis-dimers and/or small cis-oligomers in the cell junction regions. Found as dimer in the solution. Predominantly localized to the lateral cell membranes {ECO:0000250\|UniProtKB:P16573} [Isoform 3]: Secreted [Isoform 5]: Cell membrane; Single-pass type I membrane protein [Isoform 7]: Cell membrane; Single-pass type I membrane protein Cell projection, microvillus membrane {ECO:0000250\|UniProtKB:P31809}; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein. Note=Localized to the apical glycocalyx surface (PubMed:10436421). Colocalizes with CEACAM20 at the apical brush border of intestinal cells {ECO:0000250\|UniProtKB:P31809, ECO:0000269\|PubMed:10436421}
Tissue Location	Expressed in columnar epithelial cells of the colon (at protein level) (PubMed:10436421). The predominant forms expressed by T cells are those containing a long cytoplasmic domain (PubMed:18424730). Expressed in granulocytes and lymphocytes Leukocytes only express isoforms 6 and isoform 1 (PubMed:11994468)
Volume	50 µl

Research Areas

Citations (0)

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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

References

Hinoda Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6959-6963(1988).
Hinoda Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:1668-1668(1989).
Barnett T.R.,et al.J. Cell Biol. 108:267-276(1989).
Kuroki M.,et al.Biochem. Biophys. Res. Commun. 176:578-585(1991).
Barnett T.R.,et al.Mol. Cell. Biol. 13:1273-1282(1993).

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