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SPSB3 Antibody - C-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q6PJ21 |
| Other Accession | NM_080861, NP_543137 |
| Reactivity | Human |
| Predicted | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 39kDa |
| Gene ID | 90864 |
|---|---|
| Alias Symbol | C16orf31, SSB3 |
| Other Names | SPRY domain-containing SOCS box protein 3, SSB-3, SPSB3, C16orf31, SSB3 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 &mu, l of distilled water. Final Anti-SPSB3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | SPSB3 Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPSB3 {ECO:0000303|PubMed:38418882, ECO:0000312|HGNC:HGNC:30629} |
|---|---|
| Function | Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as CGAS and SNAI1 (PubMed:29059170, PubMed:38418882). The ECS(SPSB3) complex catalyzes 'Lys-48'-linked ubiquitination of nuclear CGAS in cycling cells, leading to its degradation (PubMed:38418882). Recognizes and binds nucleosome-bound CGAS: ubiquitination and degradation of nuclear CGAS during G1 and G2 phases is required to promote low intranuclear CGAS abundance before the next mitotic cycle (PubMed:38418882). The ECS(SPSB3) complex also mediates ubiquitination and degradation of phosphorylated SNAI1 (PubMed:29059170). |
| Cellular Location | Nucleus. Note=Nuclear SPSB3 levels increase during interphase |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
References
Friedel E.J.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Daniels R.J.,et al.Hum. Mol. Genet. 10:339-352(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Wang D.,et al.J. Biol. Chem. 280:16393-16401(2005).
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