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EIF3J antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • WB - EIF3J antibody - N-terminal region AI15212

    Host: Rabbit
    Target Name: EIF3J
    Antibody Dilution: 1.0μg/ml
    Sample Tissue: Jurkat cell lysate
    EIF3J is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O75822
Other Accession NM_003758, NP_003749
Reactivity Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog
Predicted Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 29kDa
Additional Information
Gene ID 8669
Alias Symbol EIF3S1, eIF3-alpha, eIF3-p35
Other Names Eukaryotic translation initiation factor 3 subunit J {ECO:0000255|HAMAP-Rule:MF_03009}, eIF3j {ECO:0000255|HAMAP-Rule:MF_03009}, Eukaryotic translation initiation factor 3 subunit 1 {ECO:0000255|HAMAP-Rule:MF_03009}, eIF-3-alpha {ECO:0000255|HAMAP-Rule:MF_03009}, eIF3 p35 {ECO:0000255|HAMAP-Rule:MF_03009}, EIF3J {ECO:0000255|HAMAP-Rule:MF_03009}
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-EIF3J antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsEIF3J antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3J {ECO:0000255|HAMAP-Rule:MF_03009}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03009}.
Citations (0)
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References

Block K.L.,et al.J. Biol. Chem. 273:31901-31908(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Li W.B.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:671-675(2006).
Bienvenut W.V.,et al.Submitted (MAR-2008) to UniProtKB.

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$ 389.00
Cat# AI15212
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