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> home > Products > Primary Antibodies > Antibody Collections > Fc gamma R-mediated phagocytosis > SPHK1 antibody - middle region
SPHK1 antibody - middle region
Rabbit Polyclonal Antibody
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Application 
| WB |
|---|---|
| Primary Accession | Q9NYA1 |
| Other Accession | NM_001142601, NP_001136073 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Goat, Horse, Bovine, Guinea Pig, Dog |
| Predicted | Human, Mouse, Rat, Rabbit, Chicken, Horse, Bovine, Guinea Pig, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 42kDa |
| Gene ID | 8877 |
|---|---|
| Alias Symbol | SPHK |
| Other Names | Sphingosine kinase 1, SK 1, SPK 1, 2.7.1.91, SPHK1, SPHK, SPK |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-SPHK1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | SPHK1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPHK1 (HGNC:11240) |
|---|---|
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo- dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:11923095, PubMed:20577214, PubMed:23602659, PubMed:24929359, PubMed:29662056). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF- kappa-B in response to TNF signaling leading to IL17 secretion (PubMed:20577214). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed:23935096). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:24929359, PubMed:28049734). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698). |
| Cellular Location | Cytoplasm. Nucleus. Cell membrane. Endosome membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Synapse {ECO:0000250|UniProtKB:Q8CI15} Note=Translocated from the cytoplasm to the plasma membrane in a CIB1- dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359) Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). {ECO:0000250|UniProtKB:Q8CI15, ECO:0000269|PubMed:19854831, ECO:0000269|PubMed:24929359} |
| Tissue Location | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056). |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Melendez A.J.,et al.Gene 251:19-26(2000).
Nava V.E.,et al.FEBS Lett. 473:81-84(2000).
Pitson S.M.,et al.Biochem. J. 350:429-441(2000).
Van Veldhoven P.P.,et al.Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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$ 389.00
Cat# AI14965
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