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CHST1 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - CHST1 antibody - middle region AI12584

    WB Suggested Anti-CHST1 Antibody Titration: 0.2-1 μg/ml
    Positive Control: Human kidney
    detail
  • WB - CHST1 antibody - middle region AI12584

    Host:Rabbit
    Target Name:CHST1
    Sample Tissue:Human Fetal Heart
    Antibody Dilution: 1.0μg/ml
    detail
  • WB - CHST1 antibody - middle region AI12584

    Host:Rabbit
    Target Name:CHST1
    Sample Tissue:Human Fetal Lung
    Antibody Dilution: 1.0μg/ml
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O43916
Other Accession NM_003654, NP_003645
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Zebrafish, Chicken, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 47kDa
Additional Information
Gene ID 8534
Alias Symbol C6ST, KS6ST, KSGal6ST, KSST, GST-1
Other Names Carbohydrate sulfotransferase 1, 2.8.2.21, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, KSST, CHST1
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-CHST1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsCHST1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST1 (HGNC:1969)
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of internal galactose (Gal) residues of keratan. Cooperates with B4GALT4 and B3GNT7 glycosyltransferases and CHST6 sulfotransferase to construct and elongate disulfated disaccharide unit [->3(6- sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:10642612, PubMed:17690104, PubMed:9405439). Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer (PubMed:9405439). Involved in biosynthesis of phosphacan, a major keratan sulfate proteoglycan in the developing brain (By similarity). Involved in biosynthesis of 6-sulfoGalbeta- containing O-linked glycans in high endothelial venules of lymph nodes. May act in a synergistic manner with CHST4 to generate sialyl 6',6- disulfo Lewis X motif, a recognition determinant for immune cell receptors implicated in leukocyte trafficking (PubMed:10330415). Catalyzes sulfation of N-acetyllactosamine (LacNAc) oligosaccharides with highest efficiency for sialylated LacNAc structures (PubMed:10642612).
Cellular Location Golgi apparatus membrane; Single- pass type II membrane protein
Tissue Location Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.
Research Areas
Citations (0)
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References

Kitayama,K.,(2007)J.Biol.Chem.282(41),30085-30096ReconstitutionandStorage:Forshorttermuse,storeat2-8Cupto1week.Forlongtermstorage,storeat-20Cinsmallaliquotstopreventfreeze-thawcycles.

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$ 389.00
Cat# AI12584
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