PRF1 antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P14222 |
Other Accession | NM_005041, NP_005032 |
Reactivity | Human, Rat, Rabbit, Pig, Horse, Bovine, Dog |
Predicted | Human, Pig, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 59kDa |
Gene ID | 5551 |
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Alias Symbol | FLH2, HPLH2, MGC65093, P1, PFP, PFN1 |
Other Names | Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein, PFP, PRF1, PFP |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-PRF1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | PRF1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PRF1 |
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Synonyms | PFP |
Function | Pore-forming protein that plays a key role in granzyme- mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:20889983, PubMed:21037563, PubMed:24558045, PubMed:9058810, PubMed:9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity). |
Cellular Location | Cytolytic granule. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Note=Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T- lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin- coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786). |
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Provided below are standard protocols that you may find useful for product applications.
References
Okur,H., (2008) Leuk. Res. 32 (6), 972-975 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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