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MCM4 antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P33991 |
| Other Accession | NM_182746, NP_877423 |
| Reactivity | Human, Mouse, Rat, Rabbit, Horse, Bovine, Dog |
| Predicted | Human, Mouse, Rat, Rabbit, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 95kDa |
| Gene ID | 4173 |
|---|---|
| Alias Symbol | CDC21, CDC54, hCdc21, P1-CDC21 |
| Other Names | DNA replication licensing factor MCM4, 3.6.4.12, CDC21 homolog, P1-CDC21, MCM4, CDC21 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 100 ul of distilled water. Final anti-MCM4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | MCM4 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MCM4 (HGNC:6947) |
|---|---|
| Synonyms | CDC21 |
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914). |
| Cellular Location | Nucleus. Chromosome. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
Ishimi,Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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