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TRIM21 antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P19474 |
| Other Accession | NM_003141, NP_003132 |
| Reactivity | Human, Mouse, Rat, Dog |
| Predicted | Human, Mouse, Rat, Guinea Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 54kDa |
| Gene ID | 6737 |
|---|---|
| Alias Symbol | RNF81, RO52, SSA, SSA1 |
| Other Names | E3 ubiquitin-protein ligase TRIM21, 6.3.2.-, 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RING finger protein 81, Ro(SS-A), Sjoegren syndrome type A antigen, SS-A, Tripartite motif-containing protein 21, TRIM21, RNF81, RO52, SSA1 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-TRIM21 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | TRIM21 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM21 (HGNC:11312) |
|---|---|
| Synonyms | RNF81, RO52, SSA1 |
| Function | E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2 (PubMed:16297862, PubMed:16316627, PubMed:16472766, PubMed:16880511, PubMed:18022694, PubMed:18361920, PubMed:18641315, PubMed:18845142, PubMed:19675099, PubMed:26347139). Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination (PubMed:16880511, PubMed:19675099). Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes (PubMed:16880511). A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated- form), thereby promoting its degradation by the proteasome (PubMed:16880511). Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling (PubMed:19675099). Negatively regulates IFN-beta production post-pathogen recognition by catalyzing polyubiquitin-mediated degradation of IRF3 (PubMed:18641315). Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway (PubMed:18022694). Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages (By similarity). Plays a role in the regulation of the cell cycle progression (PubMed:16880511). Enhances the decapping activity of DCP2 (PubMed:18361920). Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules (PubMed:1985094, PubMed:8666824). At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified (PubMed:8666824). The common feature of these proteins is their ability to bind HY RNAs.2 (PubMed:8666824). Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed:26347139). Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy (PubMed:26347139). Regulates also autophagy through FIP200/RB1CC1 ubiquitination and subsequent decreased protein stability (PubMed:36359729). Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'- linked ubiquitination of NMI (PubMed:26342464). During viral infection, promotes cell pyroptosis by mediating 'Lys-6'-linked ubiquitination of ISG12a/IFI27, facilitating its translocation into the mitochondria and subsequent CASP3 activation (PubMed:36426955). When up-regulated through the IFN/JAK/STAT signaling pathway, promotes 'Lys-27'-linked ubiquitination of MAVS, leading to the recruitment of TBK1 and up- regulation of innate immunity (PubMed:29743353). Mediates 'Lys-63'- linked polyubiquitination of G3BP1 in response to heat shock, leading to stress granule disassembly (PubMed:36692217). |
| Cellular Location | Cytoplasm. Cytoplasmic vesicle, autophagosome. Nucleus. Cytoplasm, P-body. Cytoplasm, Stress granule. Note=Enters the nucleus upon exposure to nitric oxide (PubMed:18361920). Localizes to small dot- or rod-like structures in the cytoplasm, called processing bodies (P-bodies) that are located underneath the plasma membrane and also diffusely in the cytoplasm (PubMed:18361920). They are located along the microtubules and are highly motile in cells (PubMed:18361920). Colocalizes with DCP2 in P-bodies (PubMed:18361920). Localizes to stress granules in response to oxidative stress (PubMed:36692217). |
| Tissue Location | Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Yamochi,T., (2008) Biochem. Biophys. Res. Commun. 370 (1), 195-199 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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$ 389.00
Cat# AI10615
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