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SMAD4 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - SMAD4 antibody - middle region AI10038
    SMAD4 antibody - middle region (AI10038) in Human U2OS cells using Western Blot
    Sample Type: Human nuclear cell extracts (30ug)
    Primary Dilution: 1:1000
    Secondary Antibody: anti-Rabbit HRP
    Secondary Dilution: 1:20000
    Image
    Submitted by:  Katarina Luciakova
    Cancer Research Institute See Customer Feedback tab for detailed information.
    detail
  • WB - SMAD4 antibody - middle region AI10038
    SMAD4 antibody - middle region (AI10038) in Human U2OS cells using Western Blot
    SMAD4 antibody - middle region (AI10038) validated by WB using U2OS nuclear extracts
    detail
  • WB - SMAD4 antibody - middle region AI10038
    SMAD4 antibody - middle region (AI10038) in Human HepG2 cells using Western Blot
    WB Suggested Anti-SMAD4 Antibody Titration: 1.25μg/ml
    ELISA Titer: 1:312500
    Positive Control: HepG2 cell lysate
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q13485
Other Accession Q13485, NP_005350, NM_005359
Reactivity Human, Mouse, Rat, Rabbit, Goat, Dog, Guinea Pig, Horse, Sheep, Bovine
Predicted Human, Mouse, Rat, Pig, Goat, Guinea Pig, Horse, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 60 kDa
Additional Information
Gene ID 4089
Alias Symbol JIP, DPC4, MADH4, MYHRS
Other Names Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Target/Specificity SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 100 ul of distilled water. Final anti-SMAD4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsSMAD4 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD4
Synonyms DPC4, MADH4
Function In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac- specific gene expression. Binds to SMAD binding elements (SBEs) (5'- GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF- beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD (PubMed:15799969). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236)
Research Areas
Citations (0)
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Background

This is a rabbit polyclonal antibody against SMAD4. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (sales@abgent.com).

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$ 339.00
Cat# AI10038
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