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Anti-CD80 (B7-1) Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC, E |
|---|---|
| Primary Accession | P33681 |
| Other Accession | 838 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | C80/1608 |
| Calculated MW | 33048 Da |
| Gene ID | 941 |
|---|---|
| Other Names | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; Costimulatory factor CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80 |
| Application Note | ELISA (For coating, order Ab without BSA); ,Immunofluorescence (1-2ug/ml); ,Flow Cytometry (0.5-1ug/million cells);,Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-CD80 (B7-1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD80 |
|---|---|
| Synonyms | CD28LG, CD28LG1, LAB7 |
| Function | Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed:10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed:12121659). Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed:36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein |
| Tissue Location | Expressed on activated B-cells, macrophages and dendritic cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.
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