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Anti-Topoisomerase II alpha Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | P11388 |
| Other Accession | 156346 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG2b, kappa |
| Clone Names | TOP2A/1361 |
| Calculated MW | 174385 Da |
| Gene ID | 7153 |
|---|---|
| Other Names | ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A |
| Application Note | Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); ,Western Blotting (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-Topoisomerase II alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TOP2A |
|---|---|
| Synonyms | TOP2 |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity). |
| Cellular Location | Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus |
| Tissue Location | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
It recognizes a 170kDa protein, which is identified as topoisomerase II. shows no cross-reaction with Topoisomerase II or I. Topo II plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II is also implicated in drug resistance of tumor cells.
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