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Anti-CD162 (Selectin P Ligand) Antibody

Mouse Monoclonal Antibody

     
  • IHC-P - Anti-CD162 (Selectin P Ligand) Antibody AH13508-20
    Formalin-fixed, paraffin-embedded human Spleen stained withCD162 Monoclonal Antibody (PSGL1/1601).
    detail
  • IHC-P - Anti-CD162 (Selectin P Ligand) Antibody AH13508-20
    Formalin-fixed, paraffin-embedded human Tonsil stained withCD162 Monoclonal Antibody (PSGL1/1601).
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, FC
Primary Accession Q14242
Other Accession 591014
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names PSGL1/1601
Calculated MW 43201 Da
Additional Information
Gene ID 6404
Other Names CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; PSGL1; Selectin P ligand; SELPLG
Application Note Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-CD162 (Selectin P Ligand) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SELPLG
Function A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Cellular Location Membrane; Single-pass type I membrane protein.
Tissue Location Expressed on neutrophils, monocytes and most lymphocytes
Research Areas
Citations (0)
citation

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Background

CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.

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$ 219.00
$ 499.00
Cat# AH13508-20
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