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Anti-Prostate Specific Acid Phosphatase (PSAP) Antibody

Mouse Monoclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC
Primary Accession P15309
Other Accession 433060
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names ACPP/1339
Calculated MW 44566 Da
Additional Information
Gene ID 55
Other Names 5'-nucleotidase (5'-NT); Acid phosphatase prostate; ACP3; Ecto-5'-nucleotidase; Prostatic acid phosphatase (PAP); Prostatic acid phosphatase; Thiamine monophosphatase (TMPase)
Application Note Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); ,Immunohistology (Frozen only) (0.5-1.0ug/ml for 30 minutes at RT) ,Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-Prostate Specific Acid Phosphatase (PSAP) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACP3 (HGNC:125)
Synonyms ACPP
Function A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042).
Cellular Location [Isoform 1]: Secreted
Tissue Location Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues
Research Areas
Citations (0)
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Background

Recognizes a protein of 52kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

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$ 219.00
$ 499.00
Cat# AH13462-20
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