Anti-GCDFP-15 Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, FC |
---|---|
Primary Accession | P12273 |
Other Accession | 99949 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse / IgG2a, kappa |
Clone Names | PIP/1571 |
Calculated MW | 16572 Da |
Gene ID | 5304 |
---|---|
Other Names | BRST-2; GCDFP-15; gp17; GPIP4; Gross cystic disease fluid protein 15; Prolactin-induced protein (PIP); Prolactin-inducible protein (PIP); Secretory actin-binding protein (SABP) |
Application Note | Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris buffer with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
Precautions | Anti-GCDFP-15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PIP |
---|---|
Synonyms | GCDFP15, GPIP4 |
Cellular Location | Secreted. |
Tissue Location | Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands |
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Provided below are standard protocols that you may find useful for product applications.
Background
It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
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