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Anti-Galectin-1 / Human Placental Lactogen (hPL) Antibody

Mouse Monoclonal Antibody

     
  • IHC-P - Anti-Galectin-1 / Human Placental Lactogen (hPL) Antibody AH13361-20
    Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).
    detail
  • WB - Anti-Galectin-1 / Human Placental Lactogen (hPL) Antibody AH13361-20
    Western Blot of HeLa, A431, K562 and 293 Cell Lysates using Galectin-1 Monoclonal Antibody (GAL1/1831).
    detail
Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P09382
Other Accession 445351
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, lambda
Clone Names GAL1/1831
Calculated MW 14716 Da
Additional Information
Gene ID 3956
Other Names 14kDa laminin-binding protein; Beta-galactoside-binding lectin L-14-I; Gal-1; GAL1; Galaptin; Galectin-1; HLBP14; HPL; Lactose-binding lectin 1; Lect14; Lectin galactoside-binding soluble 1; LGALS1; MAPK activating protein MP12; S-Lac lectin 1
Application Note ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); ,Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); ,Western Blotting (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-Galectin-1 / Human Placental Lactogen (hPL) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LGALS1 (HGNC:6561)
Function Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed:24752896).
Cellular Location Secreted, extracellular space, extracellular matrix. Cytoplasm. Secreted Note=Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum- Golgi intermediate compartment) followed by vesicle entry and secretion.
Tissue Location Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Research Areas
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$ 239.00
Cat# AH13361-20
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