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CD20 / MS4A1 (B-Cell Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone 109-3C2 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC |
|---|---|
| Primary Accession | P11836 |
| Other Accession | 931, 712553 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG3, kappa |
| Clone Names | 109-3C2 |
| Calculated MW | 33-37kDa |
| Gene ID | 931 |
|---|---|
| Other Names | B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | CD20 / MS4A1 (B-Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MS4A1 |
|---|---|
| Synonyms | CD20 |
| Function | B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor. Note=Constitutively associated with membrane rafts. |
| Tissue Location | Expressed on B-cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a protein of 30-33kDa, which is identified as CD20 (Workshop V; Code CD20.12. Workshop IV; Code B17). It recognizes an extracellular domain of CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.
References
Schlossman S, et al. (eds). Leukocyte Typing V, Oxford University Press, Oxford, p511-515, 1995. | Knapp W et al. (eds) Leukocytes Typing IV, Oxford University Press, Oxford, p51, 1989. | Tedder TF and Schlossman SF. Phosphorylation of the B1 (CD20) molecule by normal and malignant human B-lymphocytes. J Biol Chem 1988, 263(20):10009-10015. | Bubien JK et al. Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B-lymphocytes. J Cell Biol 1993, 121(5):1121-1132. | Tedder TF and Engel P. CD20: a regulator of cell-cycle progression of B-lymphocytes. Immunol Today 1994, 15(9):450-454. | Kanzaki M et al. Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells. J Biol Chem 1995, 270(22):13099-13104
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