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Tyrosinase (Melanoma Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone OCA1/812 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, FC, E |
|---|---|
| Primary Accession | P14679 |
| Other Accession | 7299, 503555 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG2a, kappa |
| Clone Names | OCA1/812 |
| Calculated MW | 70-80kDa |
| Gene ID | 7299 |
|---|---|
| Other Names | Tyrosinase, 1.14.18.1, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Tyrosinase (Melanoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TYR (HGNC:12442) |
|---|---|
| Function | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4- dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA- quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:28661582). |
| Cellular Location | Melanosome membrane; Single-pass type I membrane protein. Melanosome {ECO:0000250|UniProtKB:P11344}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38 {ECO:0000250|UniProtKB:P11344} |
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Background
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
References
Korner, A., et al. 1982. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin. Science 217: 1163-1165. | Hearing, V.J., et al. 1987. Mammalian tyrosinase—the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19: 1141-1147
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