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GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody - With BSA and Azide
Rat Monoclonal Antibody [Clone SPM249 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | P14625 |
| Other Accession | 7184, 192374 |
| Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Bovine, Sheep, Xenopus, Horse, Guinea Pig, Dog |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | Rat / IgG2a, kappa |
| Clone Names | SPM249 |
| Calculated MW | 94kDa |
| Gene ID | 7184 |
|---|---|
| Other Names | Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HSP90B1 (HGNC:12028) |
|---|---|
| Function | Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum- Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059). |
| Cellular Location | Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen {ECO:0000250|UniProtKB:P41148}. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
References
Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987). | Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990). |
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