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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Receptors – Sialic acid   >    gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody - With BSA and Azide   

gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone HMB45 + PMEL/783 ]

     
  • IHC -  gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody - With BSA and Azide AH12312-20
    Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 / Melanosome Monoclonal Antibody (HMB45 + PMEL/783).
    detail
  • IHC -  gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody - With BSA and Azide AH12312-20
    Formalin-fixed, paraffin-embedded human Testis stained with gp100 / Melanosome Monoclonal Antibody (HMB45 + PMEL/783).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC, IF, FC
Primary Accession P40967
Other Accession 6490, 95972
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG's
Clone Names HMB45 + PMEL/783
Calculated MW 90-100kDa
Additional Information
Gene ID 6490
Other Names Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95 kDa melanocyte-specific secreted glycoprotein, P26, Secreted melanoma-associated ME20 antigen, ME20-S, ME20S, M-beta, PMEL, D12S53E, PMEL17, SILV
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PMEL
Synonyms D12S53E, PMEL17, SILV
Function Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein. Endosome, multivesicular body. Melanosome Extracellular vesicle. Secreted. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes (PubMed:11694580, PubMed:12643545). Sorted to stage I melanosomes following its processing in the ER and cis-Golgi (PubMed:15096515) Transiently expressed at the cell surface before targeting to early melanosomes (PubMed:16760433, PubMed:30988362). Colocalizes with BACE2 in stage I and II melanosomes (PubMed:23754390). Colocalizes with CD63 and APOE at exosomes and in intraluminal vesicles within multivesicular endosomes (PubMed:21962903, PubMed:26387950)
Tissue Location Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Overexpressed in melanomas. Some expression was found in dysplastic nevi.
Research Areas
Citations (0)
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Background

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

References

Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203

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$ 219.00
$ 499.00
Cat# AH12312-20
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