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Cytochrome C (Mitochondrial Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone 6H2.B4 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC |
|---|---|
| Primary Accession | P99999 |
| Other Accession | 54205, 437060 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | 6H2.B4 |
| Calculated MW | 15kDa |
| Gene ID | 54205 |
|---|---|
| Other Names | Cytochrome c, CYCS, CYC |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Cytochrome C (Mitochondrial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CYCS |
|---|---|
| Synonyms | CYC |
| Function | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. |
| Cellular Location | Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
References
Goshorn SG, E Retzel, and R Jemmerson. Common Structural Features among Monoclonal Antibodies Binding the Same Antigenic Region of Cytochrome c. J Biol Chem 266:2134-2142 (1991)
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