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Interferon gamma (IFNG) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM408 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC |
|---|---|
| Primary Accession | P01579 |
| Other Accession | 3458, 856 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | SPM408 |
| Calculated MW | 20-25kDa |
| Gene ID | 3458 |
|---|---|
| Other Names | Interferon gamma, IFN-gamma, Immune interferon, IFNG |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Interferon gamma (IFNG) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IFNG |
|---|---|
| Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity). |
| Cellular Location | Secreted. |
| Tissue Location | Released primarily from activated T lymphocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN- and recognizes both recombinant and native human IFN-γ. It does not neutralize the activity of IFN-. T lymphocytes and NK cells mainly produce IFN-. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN- (type I interferon).
References
Vilcek J. Forty years of interferon, forty years of cytokines. Cytokine Growth Factor Rev 1997,8(4):239 | Farrar MA and Schreiber RD. The molecular cell biology of interferon-gamma and its receptor. Annu Rev Immunol 1993, 11:571-611 | Vilcek J et al. Induction of human interferon gamma with phorbol esters and phytohemagglutinin. Methods Enzymol 1986,119:48-54 |
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