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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Interleukin signaling   >    p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - With BSA and Azide   

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone DCS-72.F6 + KIP1/769 ]

     
  • IHC -  p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - With BSA and Azide AH11013-20
    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, FC
Primary Accession P46527
Other Accession 1027, 238990
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG's
Clone Names DCS-72.F6 + KIP1/769
Calculated MW 25-26kDa
Additional Information
Gene ID 1027
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN1B {ECO:0000303|PubMed:20824794}
Function Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity)
Tissue Location Expressed in kidney (at protein level) (PubMed:15509543). Expressed in all tissues tested (PubMed:8033212) Highest levels in skeletal muscle, lowest in liver and kidney (PubMed:8033212).
Research Areas
Citations (0)
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Background

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

References

Fredersdorf S et. al. Proc Natl Acad Sci 1997;94:6380-5

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$ 219.00
$ 499.00
Cat# AH11013-20
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