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TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM542 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, FC |
|---|---|
| Primary Accession | P01135 |
| Other Accession | 7039, 170009 |
| Reactivity | Human, Rabbit, Zebrafish |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgM, kappa |
| Clone Names | SPM542 |
| Calculated MW | ~6kDa |
| Gene ID | 7039 |
|---|---|
| Other Names | Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TGFA |
|---|---|
| Function | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. |
| Cellular Location | [Transforming growth factor alpha]: Secreted, extracellular space |
| Tissue Location | Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
References
Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993, 134(24):1303-7. Language: Hungarian. | Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35. | Nasim, M. M., Thomas, D. M., Alison, M. R., and Fil]ipe, M. I. Transforming growth factor a expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma – an immunohistochemical study, Histopathology. 20: 339-343, 1992
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