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Ep-CAM / CD326 (Epithelial Marker)Mouse Monoclonal Antibody [Clone EGP40/837]

Purified Mouse Monoclonal Antibody

     
  •  - Ep-CAM / CD326 (Epithelial Marker)Mouse Monoclonal Antibody [Clone EGP40/837] AH10372-50
    Flow Cytometric analysis of human Ep-CAM on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Ep-CAM MAb (EGP40/837).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Primary Accession P16422
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names EGP40/837
Calculated MW 40-43kDa
Additional Information
Gene ID 4072
Other Names Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2
Target/Specificity Recombinant full-length human TACSTD1 protein
Application Note Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Format 0.5ml at 100ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsEp-CAM / CD326 (Epithelial Marker)Mouse Monoclonal Antibody [Clone EGP40/837] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPCAM
Synonyms GA733-2, M1S2, M4S1, MIC18, TACSTD1, TRO
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Cellular Location Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction
Tissue Location Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC) Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Research Areas
Citations (0)
citation

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References

Bjork, P., Jonsson, U., Svedberg, H., Larsson, K., Lind, P., Dillner, J., Hedlund, G., Dohlsten, M. and Kalland, T. 1993. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glyco- protein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241.

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$ 429.00
Cat# AH10372-50
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