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Goat Anti-GDE1 / MIR16 Antibody (internal region)

Purified Goat Polyclonal Antibody

     
  • WB - Goat Anti-GDE1 / MIR16 Antibody (internal region) AF4255a
    AF4255a (0.3 µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NZC3
Other Accession 56209(mouse), 60418(rat), NP_057725.1
Reactivity Mouse, Rat
Predicted Human, Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 37718 Da
Additional Information
Gene ID 51573
Other Names GDE1; glycerophosphodiester phosphodiesterase 1; 363E6.2; MIR16; RGS16-interacting membrane protein; membrane interacting protein of RGS16; membrane-interacting protein of RGS16
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence C-SLSHTGDGKPRYD , from the internal region of the protein sequence according to NP_057725.1.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-GDE1 / MIR16 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GDE1 (HGNC:29644)
Function Hydrolyzes the phosphodiester bond of glycerophosphodiesters such as glycerophosphoinositol (GroPIns) and glycerophosphoethanolamine (GroPEth), to yield a glycerol phosphate and an alcohol (By similarity). Hydrolyzes glycerophospho-N-acylethanolamines to N- acylethanolamines in the brain and participates in bioactive N- acylethanolamine biosynthesis such as anandamide (an endocannabinoid), N-palmitoylethanolamine (an anti-inflammatory), and N- oleoylethanolamine (an anorexic). In addition, has a lysophospholipase D activity by hydrolyzing N-acyl-lysoplasmenylethanolamine (N-acyl- lysoPlsEt) to N-acylethanolamine. However lysophospholipase D activity is lower than glycerophosphodiester phosphodiesterase activity (By similarity). Has little or no activity towards glycerophosphocholine (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q9JL55}; Multi-pass membrane protein. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q9JL55}; Multi-pass membrane protein. Note=Perinuclear vesicles and cell membrane {ECO:0000250|UniProtKB:Q9JL55}
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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References

Genomic organization, characterization, and molecular 3D model of GDE1, a novel mammalian glycerophosphoinositol phosphodiesterase. Bachmann AS, Duennebier FF, Mocz G. Gene 2006 Apr 371 (1): 144-53.

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$ 341.00
Cat# AF4255a
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