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Goat Anti-ATG4A Antibody (internal region)

Purified Goat Polyclonal Antibody

     
  • IHC-P - Goat Anti-ATG4A Antibody (internal region) AF4214a
    AF4214a (2 µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC, E
Primary Accession Q8WYN0
Other Accession NP_443168.2, NP_840054.1
Reactivity Human
Predicted Human
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 45378 Da
Additional Information
Gene ID 115201
Other Names ATG4A; ATG4 autophagy related 4 homolog A (S. cerevisiae); APG4A; AUTL2; APG4 autophagy 4 homolog A; AUT-like 2, cysteine endopeptidase; OTTHUMP00000062893; autophagin 2; autophagy-related cysteine endopeptidase 2
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence TEENGTVNDQTFHC, from the internal region of the protein sequence according to NP_443168.2; NP_840054.1.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-ATG4A Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG4A {ECO:0000303|Ref.20, ECO:0000312|HGNC:HGNC:16489}
Function Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:12473658, PubMed:15169837, PubMed:17347651, PubMed:21177865, PubMed:21245471, PubMed:22302004, PubMed:32732290). The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine (PubMed:12473658, PubMed:15169837, PubMed:17347651, PubMed:21177865, PubMed:21245471, PubMed:22302004). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (PubMed:12473658, PubMed:15169837, PubMed:17347651, PubMed:21177865, PubMed:21245471, PubMed:22302004). Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP (PubMed:12473658, PubMed:15169837, PubMed:17347651, PubMed:21177865, PubMed:21245471, PubMed:22302004). Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating- like enzyme that removes ATG8 conjugated to other proteins, such as ATG3 (PubMed:31315929, PubMed:33773106). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed:29458288, PubMed:33909989). Catalyzes delipidation of PE- conjugated forms of ATG8 proteins during macroautophagy (PubMed:29458288, PubMed:33909989). Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity (PubMed:29458288). Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy (PubMed:33773106).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}.
Research Areas
Citations (0)
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References

The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A Scherz-Shouval R, Sagiv Y, Shorer H, Elazar Z J Biol Chem. 2003 Apr 18;278(16):14053-8. Epub 2002 Dec 7

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$ 341.00
Cat# AF4214a
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