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Goat Anti-Vimentin Antibody (C Terminus)

Purified Goat Polyclonal Antibody

     
  • WB - Goat Anti-Vimentin Antibody (C Terminus) AF4203a
    AF4203a (2 µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08670
Other Accession 22352(mouse), 81818(rat), NP_003371.2
Reactivity Human
Predicted Human, Mouse, Rat, Pig, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 53652 Da
Additional Information
Gene ID 7431
Other Names VIM; vimentin; FLJ36605
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence C-QVINETSQHHDDLE, from the C Terminus of the protein sequence according to NP_003371.2.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Vimentin Antibody (C Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM (HGNC:12692)
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
Citations (0)
citation

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References

Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis. Maraina CH, Nurdayana AK, Rusni D, Azwany Y. Int J Rheum Dis. 2010 Oct;13(4):335-9.

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$ 341.00
Cat# AF4203a
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Availability: 7-10 days
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