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ALDOA Antibody (internal region, near N-Term)

Peptide-affinity purified goat antibody

     
  • WB - ALDOA Antibody (internal region, near N-Term) AF4084a
    AF4084a (1 µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P04075
Other Accession NP_000025.1, NP_001230106.1, 226, 11674 (mouse), 24189 (rat)
Reactivity Human, Mouse, Rat
Predicted Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 39420 Da
Additional Information
Gene ID 226
Other Names Fructose-bisphosphate aldolase A, 4.1.2.13, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDOA Antibody (internal region, near N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDOA (HGNC:414)
Synonyms ALDA
Function Catalyzes the reversible conversion of beta-D-fructose 1,6- bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, may also function as scaffolding protein (By similarity).
Cellular Location Cytoplasm, myofibril, sarcomere, I band {ECO:0000250|UniProtKB:P00883}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000250|UniProtKB:P00883}. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+) {ECO:0000250|UniProtKB:P00883}
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (P_000025.1; NP_001230106.1). Reported variants represent identical protein: NP_908930.1, NP_908932.1, NP_001121089.1, NP_000025.1.

References

Human aldolase A natural mutants: relationship between flexibility of the C-terminal region and enzyme function. Esposito G, Vitagliano L, Costanzo P, Borrelli L, Barone R, Pavone L, Izzo P, Zagari A, Salvatore F. The Biochemical journal 2004 May 380 (Pt 1): 51-6. PMID: 14766013

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$ 341.00
Cat# AF4084a
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