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CREB1 (aa96-109) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CREB1 (aa96-109) Antibody (internal region) AF4008a
    AF4008a (0.1 µg/ml) staining of Jurkat (A) and NIH3T3 (B) nuclear lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P16220
Other Accession NP_004370.1, NP_604391.1, 1385, 12912 (mouse)
Reactivity Human, Mouse
Predicted Pig, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 35136 Da
Additional Information
Gene ID 1385
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCREB1 (aa96-109) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CREB1
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083}
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_004370.1; NP_604391.1).

References

Layer-specific CREB target gene induction in human neocortical epilepsy. Beaumont TL, Yao B, Shah A, Kapatos G, Loeb JA. The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Oct 32 (41): 14389-401. PMID: 23055509

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$ 341.00
Cat# AF4008a
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Availability: 7-10 days
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