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Stomatin Antibody (N-Term)

Peptide-affinity purified goat antibody

     
  • IHC - Stomatin Antibody (N-Term) AF3944a
    AF3944a (5 µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - Stomatin Antibody (N-Term) AF3944a
    AF3944a (0.1 µg/ml) staining of Human Lung lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P27105
Other Accession NP_004090.4, NP_937837.1, 2040
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 31731 Da
Additional Information
Gene ID 2040
Other Names Erythrocyte band 7 integral membrane protein, Protein 7.2b, Stomatin, STOM, BND7, EPB72
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsStomatin Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STOM (HGNC:3383)
Function Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane raft. Melanosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P54116}. Note=Localizes to juxtanuclear structure probably derived from the Golgi apparatus (PubMed:9243190) Colocalizes with cortical actin microfilaments at small plasma membrane protrusions (PubMed:9243190). Associates with alpha-granular lipid rafts (PubMed:12130500). Translocates from the alpha-granular lipid rafts to the cell membrane on thrombin activation and selectively enriched in released microvesicles (PubMed:12130500). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Tissue Location Detected in erythrocytes (at protein level). Widely expressed.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_004090.4; NP_937837.1).

References

Echinophilic proteins stomatin, sorcin, and synexin locate outside gangliosideM1 (GM1) patches in the erythrocyte membrane. Mrówczy?ska L, Salzer U, Perutková S, Igli? A, Hägerstrand H. Biochem Biophys Res Commun. 2010 Oct 22;401(3):396-400. PMID: 20858460

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$ 341.00
Cat# AF3944a
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