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ICAM1 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - ICAM1 Antibody (internal region) AF3862a
    AF3862a (2 µg/ml) staining of HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P05362
Other Accession NP_000192.2, 3383
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 57825 Da
Additional Information
Gene ID 3383
Other Names Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsICAM1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ICAM1
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Cellular Location Membrane; Single-pass type I membrane protein.
Research Areas
Citations (0)
citation

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Background

The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.

References

Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay. Fu C, Tong C, Wang M, Gao Y, Zhang Y, Lü S, Liang S, Dong C, Long M. J Biol Chem. 2011 Oct 7;286(40):34777-87. PMID: 21840991

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$ 341.00
Cat# AF3862a
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Availability: 7-10 days
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