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endophilin-A1 / SH3GL2 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - endophilin-A1 / SH3GL2 Antibody (internal region) AF3838a
    AF3838a (0.3 µg/ml) staining of Human Frontal Cortex lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - endophilin-A1 / SH3GL2 Antibody (internal region) AF3838a
    AF3838a (5 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q99962
Other Accession NP_003017.1, 6456
Reactivity Human
Predicted Pig
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 39962 Da
Additional Information
Gene ID 6456
Other Names Endophilin-A1, EEN-B1, Endophilin-1, SH3 domain protein 2A, SH3 domain-containing GRB2-like protein 2, SH3GL2, CNSA2, SH3D2A
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsendophilin-A1 / SH3GL2 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SH3GL2
Synonyms CNSA2, SH3D2A
Function Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O35179}. Membrane {ECO:0000250|UniProtKB:O35179}; Peripheral membrane protein {ECO:0000250|UniProtKB:O35179}. Early endosome {ECO:0000250|UniProtKB:Q62420}. Presynapse {ECO:0000250|UniProtKB:O35179}
Tissue Location Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum
Research Areas
Citations (0)
citation

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References

SH3GL2 gene participates in MEK-ERK signal pathway partly by regulating EGFR in the laryngeal carcinoma cell line Hep2. Shang C, Guo Y, Fu S, Fu W, Sun K. Med Sci Monit. 2010 Jun;16(6):BR168-73. PMID: 20512084

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$ 341.00
Cat# AF3838a
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