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> home > Products > Primary Antibodies > Antibody Collections > Goat Antibodies > PCNA Antibody (internal region)
PCNA Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, Pep-ELISA |
|---|---|
| Primary Accession | P12004 |
| Other Accession | NP_002583.1, 5111, 18538 (mouse), 25737 (rat) |
| Reactivity | Human, Mouse, Rat |
| Predicted | Pig, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 28769 Da |
| Gene ID | 5111 |
|---|---|
| Other Names | Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PCNA Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PCNA |
|---|---|
| Function | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737). |
| Cellular Location | Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Reported variants represent identical protein: NP_872590.1, NP_002583.1
References
Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. Jones MJ, Colnaghi L, Huang TT. EMBO J. 2011 Dec 13. PMID: 22157819
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$ 341.00
Cat# AF3816a
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