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ARF4 (aa137-150) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - ARF4 (aa137-150) Antibody (internal region) AF3806a
    AF3806a (0.5 µg/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P18085
Other Accession NP_001651.1, 378, 11843 (mouse), 79120 (rat)
Reactivity Human, Mouse
Predicted Rat, Pig
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 20511 Da
Additional Information
Gene ID 378
Other Names ADP-ribosylation factor 4, ARF4, ARF2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARF4 (aa137-150) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARF4
Synonyms ARF2
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation (PubMed:25673879).
Cellular Location Golgi apparatus. Membrane; Lipid-anchor
Research Areas
Citations (0)
citation

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References

Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration. Jang SY, Jang SW, Ko J. Cancer Lett. 2012 Jan 28;314(2):185-97. PMID: 22004728

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$ 341.00
Cat# AF3806a
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Availability: 7-10 days
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