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STAT5A Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - STAT5A Antibody (C-Term) AF3718a
    AF3718a (0.3 µg/ml) staining of K562 (A), Molt4 (B) and NIH3T3 (C) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - STAT5A Antibody (C-Term) AF3718a
    EB11392 (0.3µg/ml) staining of K562 (A), Molt4 (B) and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - STAT5A Antibody (C-Term) AF3718a
    EB11392 (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
    detail
  • IHC - STAT5A Antibody (C-Term) AF3718a
    EB11392 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, Pep-ELISA
Primary Accession P42229
Other Accession NP_003143.2, 6776, 20850 (mouse), 24918 (rat)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 90647 Da
Additional Information
Gene ID 6776
Other Names Signal transducer and activator of transcription 5A, STAT5A, STAT5
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTAT5A Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STAT5A
Synonyms STAT5
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL- induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
Citations (0)
citation

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Background

This antibody is not expected to cross-react with STAT5B.

References

Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoietin-induced signaling pathway. Okutani Y, Kitanaka A, Tanaka T, Kamano H, Ohnishi H, Kubota Y, Ishida T,
Takahara J. Oncogene. 2001 Oct 4;20(45):6643-50. PMID: 11641791

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$ 341.00
Cat# AF3718a
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Availability: 7-10 days
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