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p15INK4b (isoform 2) Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - p15INK4b (isoform 2) Antibody (C-Term) AF3669a
    AF3669a (1 µg/ml) staining of Human Gastrointestinal cancer lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P42772
Other Accession NP_004927.2, NP_511042.1, 1030
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 14722 Da
Additional Information
Gene ID 1030
Other Names Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp15INK4b (isoform 2) Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN2B
Synonyms MTS2
Function Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
Cellular Location Cytoplasm. Note=Also found in the nucleus
Tissue Location Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize isoform 2 (NP_511042.1) only.

References

Strategies to re-express epigenetically silenced p15(INK4b) and p21(WAF1) genes in acute myeloid leukemia. Geyer CR. Epigenetics. 2010
Nov-Dec;5(8):696-703. Review. PMID: 21124069

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$ 341.00
Cat# AF3669a
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Availability: 7-10 days
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