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ADAM12 (aa225-239) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - ADAM12 (aa225-239) Antibody (internal region) AF3601a
    AF3601a (0.3 µg/ml) staining of Mouse Heart lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O43184
Other Accession NP_003465.3, NP_067673.2, 8038, 11489 (mouse), 679837 (rat)
Reactivity Human, Mouse, Rat
Predicted Pig
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 99542 Da
Additional Information
Gene ID 8038
Other Names Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, 3.4.24.-, Meltrin-alpha, ADAM12, MLTN
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADAM12 (aa225-239) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADAM12
Synonyms MLTN
Function Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 3]: Secreted.
Tissue Location Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).

References

Z-DNA-forming silencer in the first exon regulates human ADAM-12 gene expression. Ray BK, Dhar S, Shakya A, Ray A. Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):103-8. PMID: 21173277

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$ 341.00
Cat# AF3601a
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