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CD56 / NCAM1 (isoform 2) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CD56 / NCAM1 (isoform 2) Antibody (internal region) AF3553a
    AF3553a (0.01 µg/ml) staining of Human Frontal Cortex lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - CD56 / NCAM1 (isoform 2) Antibody (internal region) AF3553a
    EB10948 (0.01µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - CD56 / NCAM1 (isoform 2) Antibody (internal region) AF3553a
    EB10948 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • FC - CD56 / NCAM1 (isoform 2) Antibody (internal region) AF3553a
    EB10948 Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, Pep-ELISA
Primary Accession P13591
Other Accession NP_851996.2, 4684, 24586 (rat)
Reactivity Human
Predicted Rat, Pig
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 94574 Da
Additional Information
Gene ID 4684
Other Names Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56, NCAM1, NCAM
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD56 / NCAM1 (isoform 2) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCAM1 (HGNC:7656)
Synonyms NCAM
Function This protein is a cell adhesion molecule involved in neuron- neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for Zika virus.
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 3]: Cell membrane; Lipid-anchor, GPI- anchor [Isoform 5]: Secreted.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.

References

CD56 (NCAM)
expression in ameloblastomas and other odontogenic lesions. Cairns L, Naidu A, Robinson CM, Sloan P, Wright JM, Hunter KD. Histopathology. 2010 Oct;57(4):544-8. PMID: 20875069

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$ 341.00
Cat# AF3553a
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