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ACPP / PAP (aa269-282) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - ACPP / PAP (aa269-282) Antibody (internal region) AF3540a
    AF3540a (0.01 µg/ml) staining of Human Prostate lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - ACPP / PAP (aa269-282) Antibody (internal region) AF3540a
    AF3540a (3.8 µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - ACPP / PAP (aa269-282) Antibody (internal region) AF3540a
    EB10915 (0.01µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - ACPP / PAP (aa269-282) Antibody (internal region) AF3540a
    EB10915 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, Pep-ELISA
Primary Accession P15309
Other Accession NP_001127666.1, NP_001090.2, 55
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 44566 Da
Additional Information
Gene ID 55
Other Names Prostatic acid phosphatase, PAP, 3.1.3.2, 5'-nucleotidase, 5'-NT, 3.1.3.5, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACPP / PAP (aa269-282) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACP3 (HGNC:125)
Synonyms ACPP
Function A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042).
Cellular Location [Isoform 1]: Secreted
Tissue Location Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).

References

Semen-derived amyloid fibrils drastically enhance HIV infection. Münch J, Rücker E, Ständker L, Adermann K, Goffinet C, Schindler M, Wildum S, Chinnadurai R, Rajan D, Specht A, Giménez-Gallego G, Sánchez PC, Fowler DM, Koulov A, Kelly JW, Mothes W, Grivel JC, Margolis L, Keppler OT, Forssmann WG, Kirchhoff F, Cell 2007 Dec 131 (6): 1059-71. PMID: 18083097

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$ 341.00
Cat# AF3540a
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Availability: 7-10 days
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